Journal: Journal of Virology

Article Title: Resistance of endothelial cells to SARS-CoV-2 infection in vitro

doi: 10.1128/jvi.01205-25

Figure Lengend Snippet: mRNA expression of ACE2, TMPRSS2 , BSG , PPIA , and PPIB in human nasal epithelial cells (NECs) and endothelial cells (aortic, microvascular, and blood outgrowth). Expression levels for the genes ACE2 ( A ), TMPRSS2 ( B ), BSG ( C ), PPIA ( D ), and PPIB ( E ) were obtained from aortic (AoEC), microvascular (HMVEC), and blood outgrowth (BOEC) endothelial cells and NECs. Data for each donor were normalized using the average of the housekeepers (18S and Gapdh) and analyzed using a comparative Ct method (2ΔΔCt). Data are shown as the mean ± SEM fold change compared to nasal epithelium ( n = 3 wells using cells from two donors) for AoEC ( n = 3 wells using cells of three separate donors), HMVEC ( n = 3 wells using cells of three separate donors and BOECs ( n = 2 wells using cells of two separate donors).

Article Snippet: Nasal epithelial cells (Promocell) were maintained in Airway Epithelial Growth Media (Promocell) and differentiated nasal epithelial cells (MucilAir) (Epithelix, Switzerland) were grown in air-liquid interface culture using MucilAir Culture Medium (Epithelix).

Techniques: Expressing

Journal: Journal of Virology

Article Title: Resistance of endothelial cells to SARS-CoV-2 infection in vitro

doi: 10.1128/jvi.01205-25

Figure Lengend Snippet: SARS-CoV-2 virus infection in human airway epithelial cells in air:liquid interface, Vero E6, and endothelial cells. Human airway epithelial cells grown in an air:liquid interface (MucilAir) were infected with SARS-CoV-2 live virus (MOI = 0.1). Infectious virus released to the apical side of the epithelium was determined over time (6, 24, 48, and 72 h post-infection) ( A ). In separate studies, the levels of SARS-CoV-2 nucleocapsid or spike protein in Vero E6 and endothelial cells (treated with media only [untreated] or IL-1β [10 ng/mL; 3 h]) at 24 ( B ) and 72 ( C ) h post-infection with SARS-CoV-2 (MOI = 0.1) were determined using fluorescent imaging. Mock controls (media only) experiments were run simultaneously using each endothelial cell line. Data are shown as n = 3 (pooled donors) for Mucilair cells ( A ) and n = 3 (separate donors) for human aortic (AoEC), lung microvascular (HMVEC), and blood outgrowth endothelial cells (BOECs). Data are shown as mean ± SEM for (A) and representative images shown for (B) and (C) (scale bar = 25 µm).

Article Snippet: Nasal epithelial cells (Promocell) were maintained in Airway Epithelial Growth Media (Promocell) and differentiated nasal epithelial cells (MucilAir) (Epithelix, Switzerland) were grown in air-liquid interface culture using MucilAir Culture Medium (Epithelix).

Techniques: Virus, Infection, Imaging

Journal: bioRxiv

Article Title: Development of high-affinity, single-domain protein binders for neutralizing household allergens

doi: 10.1101/2025.08.03.668213

Figure Lengend Snippet: (a) Thermostability profile of AVA-1-1-C3 as a representative protein, as determined by differential scanning fluorimetry. (b-g) Cytotoxicity of primary human cells following 48-hour exposure to AVA-1-1-C3 or an equivalent concentration of bovine serum albumin (BSA), evaluated using the CellTiter-Glo® (CTG) luminescent cell viability assay. Cell types included human epithelial keratinocytes (HEK, b), human dermal fibroblasts (HDF, c), human follicle dermal papilla cells (HFDPC, d), human renal cortical epithelial cells (HRCEPC, e), human skeletal muscle cells (HSKMC, f), and human dermal microvascular endothelial cells (HDMEC, g). No significant differences in cell viability were observed. Data in (b-g) represents mean ± s.d. and are representative of two experimental replicates.

Article Snippet: Cells were cultured in their corresponding complete media including HDF growth medium (Cell Applications, Cat# 116-500), human EpiVita serum-free growth medium (Cell Applications, Cat# 141-500a), microvascular endothelial cell growth medium (PromoCells, Cat# C-22120), HSkMC growth medium (Cell Applications, Cat# 151-500), and renal epithelial cell growth media (PromoCell, Cat# C-26130).

Techniques: Concentration Assay, Cell Viability Assay